OPTIMASI NANOSTRUCTURED LIPID CARRIER LINESTRENOL DARI CAMPURAN PALM STEARIN DAN PALM KERNEL

Ayu Rana Esadini; Iskandarsyah Iskandarsyah; Harmita Harmita

doi:10.52118/edumasda.v6i2.164

OPTIMASI NANOSTRUCTURED LIPID CARRIER LINESTRENOL DARI CAMPURAN PALM STEARIN DAN PALM KERNEL

Ayu Rana Esadini, Iskandarsyah Iskandarsyah, Harmita Harmita

Abstract

A nanostructured lipid carrier (NLC) was a lipid-based nanoparticle that was considered as a potential drug delivery carrier. Lipids as matrix of NLC can be found in natural sources, such as palm oil. Palm oil and its derivatives, such as Palm Stearin (PS) and Palm Kernel Oil (PKO), are widely available in Indonesia and can be used in the pharmaceutical sector. The main purpose of this study is to develop an optimized NLC formulation containing lynestrenol using a mixture of PS and PKO as a lipid matrix. NLC was prepared using high shear homogenization followed by the ultrasonication method. Process parameters in sonication such as duration, amplitude, and interval of sonication were optimized to obtain NLC with desirable characteristics. The optimal condition of sonication was then used to obtain NLC containing lynestrenol. The ratio of PS as solid liquid and PKO as liquid lipid used in the formulation of NLC containing lynestrenol was also optimized and evaluated for its particle size, polydispersity index (PDI), and zeta potential value. The results showed that the optimum condition of sonication was obtained with an amplitude of 20% for 4.5 minutes without intervals during the sonication process. Formula Fb-3 with ratio PS:PKO (6:4) and sonicated using obtained condition obtained desirable particle size (125.90 ± 3.24 nm), zeta potential value (-29.87 ± 1.94 mV), and PDI (0.21 ± 0.026). Therefore, the NLC obtained might be a potential delivery system for the transdermal route of lynestrenol.

ABSTRAK

Nanostructured lipid carrier (NLC) adalah salah satu nanopartikel berbasis lipid yang digunakan sebagai sistem pembawa obat. Lipid sebagai matriks penyusun NLC dapat ditemukan dari sumber alam, seperti dari minyak kelapa sawit. Minyak kelapa sawit dan turunannya, seperti fraksi minyak stearin (PS) dan fraksi inti kelapa sawit (PKO) banyak ditemukan di Indonesia dan dapat digunakan di sector farmasi. Penelitian ini bertujuan untuk optimasi formulasi NLC mengandung linestrenol, dimana NLC mengandung campuran fraksi PS dan PKO sebagai matriks lipid dari NLC. NLC dibuat dengan menggunakan metode high shear homogenization dan ultrasonikasi. Beberapa parameter sonikasi seperti durasi, amplitudo, dan interval sonikasi dioptimasi untuk mendapatkan NLC dengan karakteristik yang sesuai. Kondisi optimal dalam pembuatan NLC kemudian digunakan untuk menghasilkan NLC mengandung zat aktif linestrenol. Rasio dari PS sebagai lipid padat dan PKO sebagai lipid cair juga dioptimasi dan dilakukan evaluasi terhadap beberapa parameter seperti ukuran partikel, indeks polidispersitas, dan nilai zeta potensial. Hasil penelitian menunjukkan bahwa kondisi optimum sonikasi diperoleh dengan amplitudo 20% selama 4.5 menit tanpa adanya jeda selama proses sonikasi. Formula Fb-3 dengan rasio PS:PKO (6:4) yang disonikasi dengan kondisi optimum menghasilkan NLC dengan karakteristik yang diinginkan. Fb-3 menghasilkan ukuran partikel (125,90 ± 3,24 nm), zeta potensial (-29,87 ± 1,94 mV), dan PDI (0,21 ± 0,026). Sehingga NLC yang dihasilkan berpotensi untuk dijadikan sebagai sistem penghantar obat linestrenol melalui rute transdermal.

Keywords

Nanostructured lipid carrier;Lynestrenol;Sonication;Palm stearin;Palm kernel oil

Full Text:

PDF (Bahasa Indonesia) Hasil Cek Turnitin

References

Apostolou, M., Assi, S., Fatokun, A. A., & Khan, I. (2021). The Effects of Solid and Liquid Lipids on the Physicochemical Properties of Nanostructured Lipid Carriers. Journal of Pharmaceutical Sciences, 110(8), 2859–2872. https://doi.org/10.1016/j.xphs.2021.04.012

Babazadeh, A., Ghanbarzadeh, B., & Hamishehkar, H. (2017). Formulation of food-grade nanostructured lipid carrier (NLC) for potential applications in medicinal-functional foods. Journal of Drug Delivery Science and Technology, 39, 50–58. https://doi.org/10.1016/j.jddst.2017.03.001

Chauhan, I., Yasir, M., Verma, M., & Singh, A. P. (2020). Nanostructured Lipid Carriers: A Groundbreaking Approach for Transdermal Drug Delivery. Advanced Pharmaceutical Bulletin, 10(2), 150–117. https://doi.org/10.15171/apb.2020.021

Chauhan, M. K., & Sharma, P. K. (2019). Optimization and characterization of rivastigmine nanolipid carrier loaded transdermal patches for the treatment of dementia. Chemistry and Physics of Lipids, 224(November 2018), 104794. https://doi.org/10.1016/j.chemphyslip.2019.104794

de Melo Barbosa, R., Severino, P., Finkler, C. L. L., & de Paula, E. (2019). Lipid-based colloidal carriers for transdermal administration of bioactives. Materials for Biomedical Engineering, 369–397. https://doi.org/10.1016/b978-0-12-818433-2.00011-x

de Souza, I. D. L., Saez, V., de Campos, V. E. B., & Mansur, C. R. E. (2019). Size and Vitamin E Release of Nanostructured Lipid Carriers with Different Liquid Lipids, Surfactants and Preparation Methods. Macromolecular Symposia, 383(1), 1–7. https://doi.org/10.1002/masy.201800011

Elmowafy, M., Shalaby, K., Ali, H. M., Alruwaili, N. K., Salama, A., Ibrahim, M. F., Ahmed, T. A. (2019). Impact of nanostructured lipid carriers on dapsone delivery to the skin: in vitro and in vivo studies. International Journal of Pharmaceutics, 572(October), 118781. https://doi.org/10.1016/j.ijpharm.2019.118781

Goon, D. E., Abdul Kadir, S. H. S., Latip, N. A., Rahim, S. A., & Mazlan, M. (2019). Palm oil in lipid-based formulations and drug delivery systems. Biomolecules, 9(2), 1–19. https://doi.org/10.3390/biom9020064

Gu, Y., Tang, X., Yang, M., Yang, D., & Liu, J. (2019). Transdermal drug delivery of triptolide-loaded nanostructured lipid carriers: Preparation, pharmacokinetic, and evaluation for rheumatoid arthritis. International Journal of Pharmaceutics, 554(September 2018), 235–244. https://doi.org/10.1016/j.ijpharm.2018.11.024

Iskandarsyah, I., Masrijal, C. D. P., & Harmita, H. (2020). Effects of sonication on size distribution and entrapment of lynestrenol transferosome. International Journal of Applied Pharmaceutics, 12(Special Issue 1), 245–247. https://doi.org/10.22159/ijap.2020.v12s1.FF053

Loo, C. H., Basri, M., Ismail, R., Lau, H. L. N., Tejo, B. A., Kanthimathi, M. S., … Choo, Y. M. (2013). Effect of compositions in nanostructured lipid carriers (NLC) on skin hydration and occlusion. International Journal of Nanomedicine, 8, 13–22. https://doi.org/10.2147/IJN.S35648

Malik, D. S., & Kaur, G. (2018). Nanostructured gel for topical delivery of azelaic acid: Designing, characterization, and in-vitro evaluation. Journal of Drug Delivery Science and Technology, 47, 123–136. https://doi.org/10.1016/j.jddst.2018.07.008

Mendes, I. T., Ruela, A. L. M., Carvalho, F. C., Freitas, J. T. J., Bonfilio, R., & Pereira, G. R. (2019). Development and characterization of nanostructured lipid carrier-based gels for the transdermal delivery of donepezil. Colloids and Surfaces B: Biointerfaces, 177(February), 274–281. https://doi.org/10.1016/j.colsurfb.2019.02.007

Müller, R. H., Radtke, M., & Wissing, S. A. (2002). Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Advanced Drug Delivery Reviews, 54(SUPPL.), 131–155. https://doi.org/10.1016/S0169-409X(02)00118-7

Nagaich, U., & Gulati, N. (2016). Nanostructured lipid carriers (NLC) based controlled release topical gel of clobetasol propionate: design and in vivo characterization. Drug Delivery and Translational Research, 6(3), 289–298. https://doi.org/10.1007/s13346-016-0291-1

Rohmah, M., Choiri, S., Raharjo, S., Hidayat, C., & Martien, R. (2020). Palm stearin and olein binary mixture incorporated into nanostructured lipids carrier: Improvement food functionality for micronutrient delivery. Journal of Food Processing and Preservation, (December 2019), 1–10. https://doi.org/10.1111/jfpp.14761

Sharma, A., & Baldi, A. (2018). Nanostructured Lipid Carriers : A Review Journal. Journal of Developing Drugs, 7(2), 1–12. https://doi.org/10.4172/2329-6631.10001

Wu, P. S., Lin, C. H., Kuo, Y. C., & Lin, C. C. (2017). Formulation and Characterization of Hydroquinone Nanostructured Lipid Carriers by Homogenization Emulsification Method. Journal of Nanomaterials, 2017. https://doi.org/10.1155/2017/3282693

DOI: http://dx.doi.org/10.52118/edumasda.v6i2.164

Refbacks

There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Sekolah Tinggi Ilmu Kesehatan Kharisma Persada

Pajajaran Street Number 1 Pamulang,
South Tangerang City, Banten Province, Indonesia, 15417
Telephone: 021-74716128 / Handphone : 081384462729

Edu Masda Journal by Sekolah Tinggi Ilmu Kesehatan Kharisma Persada is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Based on a work at http://openjournal.masda.ac.id/index.php/edumasda/index.